【AI蛋白新品】新一代FGF basic：更优异的热稳定性及干细胞培养性能！

近年来，AI技术正以前所-未有的速度重构产业逻辑，在医疗、金融、制造等领域创造颠覆性神话。在这一浪潮中，ACROBiosystems百普赛斯始终坚守“质量源于设计”（Quality by Design, QbD）的核心理念，将AI深度融入重组蛋白研发全链条，并已搭建了干湿闭环AI蛋白设计平台。我们已经采用自主研发的AI模型DeepExp对IL-21序列进行智能化改造，通过优化表达系统成功开发出具有卓越稳定性的重组人IL-21高稳定突变体蛋白Human IL-21 Superior Stable Mutant Protein, premium grade（货号：IL1-H5113）。

在AI技术平台的支持下，我们的AI蛋白新品持续上新！本周，我们为您隆重推荐新品：经AI优化的FGF basic，Human FGF basic Superior Stable Mutant Protein, premium grade（货号：BFF-H5113）。

碱性成纤维细胞生长因子（FGF basic，或bFGF/FGF-2）是FGF家族重要的成员之一，其在细胞培养中扮演着至关重要的角色。特别是在干细胞（包括胚胎干细胞和诱导多能干细胞）培养领域，FGF basic不可或缺，它是维持这些细胞自我更新能力和多能性（未分化状态）的核心因子，确保干细胞能够持续分裂而不自发分化。此外，FGF basic也能影响细胞的迁移和分化命运。当与其他特定因子（如BMPs、TGF-β等）联合使用时，它可以引导干细胞或祖细胞向特定的谱系（如神经、中胚层等）分化。FGF basic通过与细胞表面的成纤维细胞生长因子受体（FGFRs）结合，激活关键的细胞内信号通路（主要是MAPK/ERK和PI3K/AKT通路），从而精密调控这些关键的细胞行为，使其成为基础研究、药物筛选、组织工程和再生医学等广泛应用中细胞培养体系的关键组分。

然而，FGF basic在应用上面临一个长期且显著的痛点：其固有的热不稳定性。这一痛点使得研究人员为维持有效的FGF basic活性水平，不得不进行繁琐的每日补充，这种频繁操作大幅增加了实验的工作量和试剂成本。我们基于AI技术平台改造的FGF basic，Human FGF basic Superior Stable Mutant Protein, premium grade（货号：BFF-H5113）显著优化了这一特性，有效解决了细胞培养过程中因蛋白降解而需频繁补加的行业难题。

我们对比评估了突变体FGF basic蛋白（以下简称为HS-FGF2）与野生型FGF basic蛋白（以下简称为WT-FGF2）的热稳定性，结果显示：在37°C模拟生理/培养条件下处理72小时后，HS-FGF2不仅保持了诱导NIH-3T3细胞增殖的活性，其蛋白残留浓度也显著高于WT-FGF2，证明其结构更稳定，不易降解。之外，也对比了HS-FGF2与WT-FGF2在iPSC培养中的表现，结果表明HS-FGF2可突破传统FGF basic的热稳定性瓶颈。在减频换液（每2天1次）条件下，HS-FGF2仍能显著促进细胞增殖并高效维持多能性，其性能与其他商业化产品相当；而WT-FGF2在减频培养时出现细胞数量锐减与多能性标志物表达大幅下降。

验证数据

• 优异的热稳定性数据

Recombinant Human FGF basic Superior Stable Mutant Protein Induces Proliferation of NIH-3T3 Mouse Fibroblast Cells. (A) Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) or (B) wild-type (WT) Human FGF basic Protein, premium grade (Cat. No. BFF-H4117), were either untreated or incubated in culture media at 37°C for 3 days. Following 3 days of treatment at 37°C, Recombinant Human FGF basic Superior Stable Mutant Protein retained activity comparable to the untreated heat-stable variant, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human FGF basic Protein exhibited significant activity loss, indicating inferior thermal stability.

100 ng/mL Superior Stability for Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113) or (B) wild-type (WT) Human FGF basic Protein, premium grade (Cat. No. BFF-H4117), were incubated in culture media at 37°C for 0h, 8h, 24h, 48h and 72h. The concentration of residual FGF2 was detected by resDetect™ Human FGF2 ELISA Kit (Residue Testing) (Cat. No. RES-A009).

Following 72h of treatment at 37°C, Recombinant Human FGF basic Superior Stable Mutant Protein retained higher concentration comparable to the WT FGF2 protein, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human FGF basic Protein exhibited significant concentration loss, indicating inferior thermal stability.

• 优异的干细胞培养数据

100 ng/mL of either Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113, designated as HS-FGF2) or (B) wild-type (WT) Human FGF basic Protein of premium grade (Cat. No. BFF-H4117, designated as WT-FGF2) was added to the iPSC culture system. The culture protocol involved daily medium replacement or a reduced feeding schedule with medium changes every 2 days. Cell counts were assessed via AO/PI staining from passage 1 to passage 3.

The results indicated that the Recombinant Human FGF basic Superior Stable Mutant Protein significantly promoted stem cell proliferation, maintaining high cell numbers even under both daily feeding and the reduced 2-day feeding regimen, with performance comparable to that of competitor T. In contrast, the WT Recombinant Human FGF basic Protein showed a marked decrease in cell numbers under the reduced 2-day feeding condition.

100 ng/mL of either Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113, designated as HS-FGF2) or (B) wild-type (WT) Human FGF basic Protein of premium grade (Cat. No. BFF-H4117, designated as WT-FGF2) was added to the iPSC culture system. The culture protocol involved daily medium replacement or a reduced feeding schedule with medium changes every 2 days. The expression of stem cell marker genes (OCT4 and SOX2) was detected by FACS and immunofluorescence in passage 3.
 The results indicated that the Recombinant Human FGF basic Superior Stable Mutant Protein significantly maintained stem cell self-renewal, maintaining high expression of stem cell markers even under both daily feeding and the reduced 2-day feeding regimens, with performance comparable to that of competitor T. In contrast, the WT Recombinant Human FGF basic Protein showed a marked decrease in stem cell gene expression under the reduced 2-day feeding condition.