热度不减的PD1通路，快速获取FACS和Cell-based ELISA的Protocol

From figure 1, Flow Cytometry assay shows that recombinant human PD-L1 (Cat.No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10ug/mL.

Figure 1

From figure 2, Flow Cytometry assay shows that recombinant human PD-L2 (Cat.No.PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1ug/mL.

Figure 2

PD-L1 (Cat.No.PD1-H5258) is validated applicable for screening of neutralizing antibody.

FACS analysis shows that the binding of PD-L1 to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-hPD-L1 antibody. The concentration of PD-L1 used is 10 μg/mL. The IC50 is 12.92 μg/mL.

Cell-based ELISA

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat.No.PD1-H5258) with an EC50 of 0.029 μg/mL.

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (Cat.No.PD2-H5251) with an EC50 of 0.018 μg/mL.

获取FACS和Cell-based ELISA检测的Protocol请直接发送邮件至：order.cn@acrobiosystems.com

除此之外，ACROBiosystems还提供PD1通路抑制剂高通量筛选试剂盒 (Cat. No. EP-101)，用于筛选免疫后得到的抗体，提高后续细胞学实验的效率。

该试剂盒基于竞争性ELISA的实验原理，固定PD-L1，同时应用ACROBiosystems的AviTag生物素标记平台生产的Biotin PD1与候选PD1通路抑制剂竞争结合PD-L1。提供96 tests和480 tests两种规格的kit，避免了您自己开发建立ELISA的标准实验、验证过程等等环节，省时省力。该试剂盒至上市以来，已经经过广大客户认可及重复购买。

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