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3922 人阅读发布时间:2020-10-09 16:34
FMC63是一种靶向人CD19的鼠源单克隆抗体,其scFv序列已被广泛运用在Anti-CD19 CAR的构造中,包括已上市的两款靶向CD19的CAR-T细胞药物:Kymriah和Yescarta[1-2]。在CAR-T产品的开发过程中,CAR的表达检测贯穿于整个前期研发和临床研究阶段,尤其在临床PK研究中,对血液样本中CAR-T细胞的增殖水平和存续时间等指标检测时,因样本中细胞组分相对复杂,含有较多的干扰性因素,容易出现强背景结果,且随着时间的推移,CAR-T细胞在血液中的含量可能会降低到极低水平,因此血液样本中CAR的检测面临重要挑战。
Anti-FMC63 scFv Antibody因其能够特异性结合Anti-CD19 CAR上FMC63 scFv的抗原识别表位并表现出较高的特异性和灵敏度而被用于FMC63 scFv来源的CAR表达检测,然而目前市场上少有商品化的Anti-FMC63 scFv Antibody产品可供使用。
为满足市场需求,ACROBiosystems开发了针对Anti-CD19 CAR临床样本检测的Anti-FMC63 scFv Antibody,解决临床样本中细胞组分复杂、CAR-T细胞含量较低、非特异性背景强等问题,更有FITC和Biotin标记的Anti-FMC63 scFv Antibody助力您的药物研发进程。
产品优势:可一步完成Anti-CD19 CAR表达检测

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No.FM3-FY45) and isotype control respectively. FITC signal was used to evaluate the binding activity (QC tested).
产品优势:可灵活搭配多种荧光标记SA进行Anti-CD19 CAR表达检测

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No.FM3-BY45) and isotype control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
产品名称:Monoclonal Anti-FMC63 scFv Antibody

2e5 of FMC63 scFv-based anti-CD19 CAR-293 cells were stained with 100 µL of the working solution of Monoclonal AntiFMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) and isotype control respectively, washed and then followed by PE anti-mouse IgG1 Antibody and analyzed with flow cytometry (QC tested).
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参考文献:
[1]http://www.kymriah.com
[2]http://www.yescarta.com.