【CelThera™】T细胞大规模培养，选它就对了！

T淋巴细胞在人体免疫系统中扮演重要的角色，利用特定类型T细胞的固有功能来对抗疾病已成为革命性的技术手段。常见的T cell-based therapy有CAR-T疗法、TCR-T疗法等，这些疗法在患者中产生了持久且有效的临床反应。然而，T细胞的体外大量快速扩增仍是这一领域最大的挑战之一。

T细胞培养基作为T细胞体外培养关键的试剂，其可通过模拟体内微环境，使得分离后的T细胞在体外环境正常扩增，并维持其固有结构和功能。为了保证免疫细胞治疗产品的安全性和质量，国家药品监督管理局药品审评中心在2020年9月30日发布的《免疫细胞治疗产品药学研究与评价技术指导原则（征求意见稿）》中指出：尽可能选择GMP级别、无血清、无动物源性、成分确定的培养基。对于细胞治疗领域的客户来说，培养基除需满足以上特点外，还需要有良好的性能来支持T细胞的体外大量快速扩增。

• 6孔板体系下的PBMC来源T细胞培养方案

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR. Notably, the cells exhibits better expansion in CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101).

Human PBMCs were cultured with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101) or T cell culture medium (Competitor L +2.5% SR) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium (ACROBiosystems) can be comparable with the Competitor L +2.5% SR.

• T225瓶体系下的PBMC来源T细胞培养方案

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can promote the expansion of these cells with a reasonable cell viability.

Human PBMCs were cultured with GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) with CelThera™ GMP T Cell Expansion Medium (ACROBiosystems, Cat. No. GMP-CM3101 & GMP-CM3101-1) for two weeks. The result shows that CelThera™ GMP T Cell Expansion Medium with GMP Human IL-2 Protein can increase the percentage of the CD3+ cells. With the post-culture time, the proportion of CD4 cells gradually decreased, and the proportion of CD8 cells gradually increased.

• 3L体系下的PBMC来源T细胞培养方案

Human PBMCs were activated using 0.2 µg/mL GMP Monoclonal Anti-Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824), cultured with CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The results showed that GMP human IL-2 protein, GMP monoclonal anti-human CD3 antibody (OKT3), GMP monoclonal anti-human CD28 antibody and CelThrea™ GMP T cell expansion medium could be used to culture T cells in a 3L large system. It can efficiently expand cells with high viability.

Human CD3 Antibody (OKT3) (ACROBiosystems, Cat. No. GMP-MC0323) and 1 µg/mL GMP Monoclonal Anti-Human CD28 Antibody (ACROBiosystems, Cat. No. GMP-MC2824) and cultured in CelThera™ GMP T Cell Expansion culture medium (ACROBiosystems, Cat. No. GMP-CM3101) supplemented with 500 IU/mL GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) for two weeks. The result shows that percentage of CD3+ cells in living cells is above 99%. At the end of the culture process, the majority memory phenotype of T cells is TN/SCM, followed by TCM, which means that the cell harvest will have a promising effect, such as sustained proliferation, prolonged survival, and so on.