内吞新品体验计划 | pHintra™抗体标记试剂盒试用装开放申请

pHintra™抗体标记试剂盒采用氨基共价标记策略，通过抗体表面的伯胺基团（-NH₂）将pH敏感荧光染料（Em660）高效偶联至抗体，仅需离心柱纯化即可获得标记抗体，可直接用于流式细胞术或细胞成像技术的抗体内吞检测。其染料本身的pH响应特性使标记抗体随受体介导的内吞进入内体/溶酶体等酸性胞内环境时，染料被激活并发出明亮荧光。只有真正完成内吞的抗体才会产生信号。

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• 标记抗体成功应用于内吞检测-FACS

Anti-Her2 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). SK-BR-3 cells were treated with Dye Labeled Anti-Her2 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.

Anti-CD20 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). Raji cells were treated with Dye Labeled Anti-CD20 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.

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