新品上线｜pHintra™抗体标记试剂盒：快速构建抗体内吞检测工具

在 ADC药物研发与抗体功能研究中，内吞效率与胞内转运过程是成药性评价的核心指标，不同实验阶段对荧光标记技术的需求各有侧重：高通量样本筛选更看重操作便捷性，特殊物种及特殊亚型抗体研究则对适配范围与标记稳定性有更高要求。

针对不同内吞检测场景，ACROBiosystems百普赛斯推出全新pHintra^TM抗体标记试剂盒。该产品采用氨基共价标记策略，可实现pH敏感荧光染料与抗体的高效偶联，兼容多物种、多亚型lgG抗体。仅需离心柱纯化即可获得标记抗体，可直接用于基于流式细胞术或细胞成像技术等的抗体内吞检测实验，为抗体及ADC研究提供更加灵活的检测工具。

pHintra^TM抗体标记试剂盒提供了一种快速简便的方法，通过抗体表面的伯胺基团将pH敏感染料共价标记到抗体上，制备的标记抗体可以使用流式细胞术或细胞成像技术等多种方法检测抗体内吞效率。

本试剂盒使用的pH敏感染料（Em660）能在酸性环境中发出明亮荧光信号，且能显著降低背景噪声。该pH敏感染料的激发波长为643nm，发射波长为660nm。

pHintra^TM抗体标记试剂盒的技术原理图

Anti-Her2 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). SK-BR-3 cells were treated with Dye Labeled Anti-Her2 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.

Anti-CD20 Ab and Human IgG1 isotype control were labeled using pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Cat. No. ALK-A004). Raji cells were treated with Dye Labeled Anti-CD20 Ab and Dye Labeled Isotype Control separately at 37℃ for 4 hours, then analysis by Flow cytometric. APC signal was used to evaluate the internalization signal.